Hotspot

rps15-ycf1

SSC · 123445-123765 · 321 bp

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Pi 0.0469

MarkerSeek score 68.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 321
variable_sites: 22
pi: 0.0469
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	TTGAAAGAGGCGAGATGAGC	50.0	50.0	58.0	58.0	1087-1112 bp	1.0	84.2
rps15-ycf1_p2	GCTGGCCCTTTTAGGAACTT	TTGAAAGAGGCGAGATGAGC	50.0	50.0	58.1	58.0	1567-1592 bp	1.0	82.9
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	TTCTCACAAGGTATTCGCGG	50.0	50.0	58.0	58.0	1216-1223 bp	0.666667	69.0
rps15-ycf1_p4	GCTGGCCCTTTTAGGAACTT	TTCTCACAAGGTATTCGCGG	50.0	50.0	58.1	58.0	1696-1703 bp	0.666667	68.0
rps15-ycf1_p5	AGCTAAATAATCCCACCGCG	TTCTCACAAGGTATTCGCGG	50.0	50.0	58.1	58.0	1621-1628 bp	0.666667	67.7