Hotspot

ccsA-ndhD

SSC · 115316-115564 · 249 bp

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Pi 0.0601

MarkerSeek score 78.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 249
variable_sites: 19
pi: 0.0601
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTATTTTGGCGTCA	AGCAAGTGACAAGGTCGAAT	40.9	45.0	58.2	57.4	339-370 bp	1.0	66.4
ccsA-ndhD_p2	TGCTATTTTGGCGTCAATCT	AGCAAGTGACAAGGTCGAAT	40.0	45.0	55.7	57.4	333-364 bp	1.0	65.7
ccsA-ndhD_p3	TGCTATTTTGGCGTCAATCTT	AGCAAGTGACAAGGTCGAAT	38.1	45.0	56.5	57.4	333-364 bp	1.0	64.0
ccsA-ndhD_p4	TGGATATGCTATTTTGGCGTCA	GCAAGTGACAAGGTCGAATC	40.9	50.0	58.2	57.1	338-369 bp	1.0	63.7
ccsA-ndhD_p5	GGATATGCTATTTTGGCGTCA	AGCAAGTGACAAGGTCGAAT	42.9	45.0	56.5	57.4	338-369 bp	1.0	63.6