Hotspot

ndhF-rpl32

SSC · 112266-113130 · 865 bp

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Pi 0.0518

MarkerSeek score 81.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 865
variable_sites: 59
pi: 0.0518
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTTGTTGCGGTCGGAAAAAG	TCAAGTTGGTGATGGGGAAA	45.0	45.0	57.8	56.9	1230-1292 bp	1.0	79.2
ndhF-rpl32_p2	GGTCGTGTGAACCAAAAACC	TCAAGTTGGTGATGGGGAAA	50.0	45.0	57.8	56.9	1635-1697 bp	1.0	78.0
ndhF-rpl32_p3	GTCCACATACGTCGAAGGTT	TCAAGTTGGTGATGGGGAAA	50.0	45.0	57.6	56.9	1251-1313 bp	1.0	77.8
ndhF-rpl32_p4	TTTGTTGCGGTCGGAAAAAG	ATCAAGTTGGTGATGGGGAA	45.0	45.0	57.8	56.7	1231-1293 bp	1.0	77.3
ndhF-rpl32_p5	CGGTCGGAAAAAGTGGAAGT	TCAAGTTGGTGATGGGGAAA	50.0	45.0	58.4	56.9	1245-1285 bp	0.666667	60.1