Hotspot

psaI-ycf4

LSC · 58666-59080 · 415 bp

Back to Dontostemon

Pi 0.0289

MarkerSeek score 72.20

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 17
pi: 0.0289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCACTTGCAATGGCTTCTTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	57.8	58.0	527-530 bp	1.0	81.7
psaI-ycf4_p2	AGTAGGCCTAGTCTTTCCGG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.2	58.0	546-549 bp	1.0	81.1
psaI-ycf4_p3	CCTAGTCTTTCCGGCACTTG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.3	58.0	540-543 bp	1.0	80.7
psaI-ycf4_p4	GCACTTGCAATGGCTTCTTT	ACTTGTTTTTCGAGACCCCG	45.0	50.0	57.8	58.4	529-532 bp	1.0	78.8
psaI-ycf4_p5	GCACTTGCAATGGCTTCTTT	CTTGTTTTTCGAGACCCCGT	45.0	50.0	57.8	58.4	528-531 bp	1.0	78.8