Hotspot

psbG-ndhC

LSC · 49114-49197 · 84 bp

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Pi 0.0615

MarkerSeek score 72.50

Primer available yes

Label psbG-ndhC

Feature Metadata

label_name: psbG-ndhC
feature_type: igs
region: LSC
length_bp: 84
variable_sites: 6
pi: 0.0615
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG-ndhC_p1	GTTGTTCGATCAAGTACGGGA	CATGGCGAAAAGGAGCATTG	47.6	50.0	58.0	58.1	133-154 bp	1.0	78.4
psbG-ndhC_p2	TGTTGTTCGATCAAGTACGGG	CATGGCGAAAAGGAGCATTG	47.6	50.0	58.3	58.1	134-155 bp	1.0	76.2
psbG-ndhC_p3	TGTTCGATCAAGTACGGGAA	CATGGCGAAAAGGAGCATTG	45.0	50.0	56.6	58.1	131-152 bp	1.0	75.6
psbG-ndhC_p4	TTGTTCGATCAAGTACGGGA	CATGGCGAAAAGGAGCATTG	45.0	50.0	56.6	58.1	132-153 bp	1.0	75.6
psbG-ndhC_p5	GTTGTTCGATCAAGTACGGG	CATGGCGAAAAGGAGCATTG	50.0	50.0	56.6	58.1	133-154 bp	1.0	75.3