Hotspot

trnF-UUC-ndhJ

LSC · 47267-47857 · 591 bp

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Pi 0.0319

MarkerSeek score 76.30

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 28
pi: 0.0319
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	691-703 bp	1.0	86.5
trnF-UUC-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	690-702 bp	1.0	86.5
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	688-700 bp	1.0	84.9
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	694-706 bp	1.0	84.0
trnF-UUC-ndhJ_p5	AATCCTCGTGTCACCAGTTC	CCGAAAGTTGGATAGGGTGG	50.0	55.0	57.5	58.0	693-705 bp	1.0	83.2