Hotspot

psbC-trnS-UCA

LSC · 34300-34491 · 192 bp

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Pi 0.0358

MarkerSeek score 77.20

Primer available yes

Label psbC-trnS-UCA

Feature Metadata

label_name: psbC-trnS-UCA
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 10
pi: 0.0358
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UCA_p1	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	317-325 bp	1.0	82.0
psbC-trnS-UCA_p2	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	367-375 bp	1.0	81.5
psbC-trnS-UCA_p3	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	278-286 bp	1.0	81.3
psbC-trnS-UCA_p4	CGGCAGCAGGATTTGAAAAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.6	58.2	328-336 bp	1.0	80.6
psbC-trnS-UCA_p5	TTCGTGGGTCATTTATGGCA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.5	58.2	368-376 bp	1.0	79.5