Hotspot

rps15

SSC · 122109-122387 · 279 bp

Back to Dolomiaea

Pi 0.274

MarkerSeek score 69.40

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 129
pi: 0.274
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.0	58.0	1044-1060 bp	0.5	60.3
rps15_p2	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	907-923 bp	0.5	59.9
rps15_p3	GCAGTGACTCCTCCAATACG	AATAATGGGAGCCGCTTGAG	55.0	50.0	58.1	58.0	1326-1342 bp	0.5	59.7
rps15_p4	AGGAGTCTGTGCCCCAATAT	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.1	58.0	1222-1238 bp	0.5	59.5
rps15_p5	TCCCAACGCGTTACATAAGG	CAGATGGACATATGCGCTGT	50.0	50.0	58.0	58.1	892-908 bp	0.5	59.4