Hotspot

rpl32

SSC · 112152-112316 · 165 bp

Back to Dolomiaea

Pi 0.263

MarkerSeek score 67.20

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 165
variable_sites: 73
pi: 0.263
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	AGGTGTATTCTTTCCTCGAGT	CACCACCACAATGTGAGTGA	42.9	50.0	56.1	58.0	852-858 bp	0.5	37.9
rpl32_p2	AGGTGTATTCTTTCCTCGAGT	AATAAGACTCCCCACCACCA	42.9	50.0	56.1	57.9	864-870 bp	0.5	37.8
rpl32_p3	AGGTGTATTCTTTCCTCGAGT	TCTGCCCTTCTCGAGAAAGA	42.9	50.0	56.1	58.1	920-926 bp	0.5	37.7
rpl32_p4	AGGTGTATTCTTTCCTCGAGT	AGTTTCTTAGGGTGTCCGGT	42.9	50.0	56.1	58.3	1341-1359 bp	0.5	37.0
rpl32_p5	AGGTGTATTCTTTCCTCGAGTT	CACCACCACAATGTGAGTGA	40.9	50.0	56.8	58.0	852-858 bp	0.5	35.8