Hotspot

psaC

SSC · 116227-116472 · 246 bp

Back to Dolomiaea

Pi 0.248

MarkerSeek score 58.90

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 102
pi: 0.248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCTTTCCTCCCCACATGGAT	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	942 bp	0.5	59.6
psaC_p2	CTCCCGCCGTGTACAAAATA	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	987 bp	0.5	59.5
psaC_p3	CTCCCGCCGTGTACAAAATA	CTCGGGGAACTCATCGATTC	50.0	55.0	58.0	57.9	983 bp	0.5	59.2
psaC_p4	TCTTTCCTCCCCACATGGAT	CTCGGGGAACTCATCGATTC	50.0	55.0	58.0	57.9	938 bp	0.5	59.2
psaC_p5	TTCCAAAGCCACAGGATACG	AAAACTCGGGGAACTCATCG	50.0	50.0	57.9	57.9	1097 bp	0.5	58.9