Hotspot

ndhF

SSC · 108876-111110 · 2235 bp

Back to Dolomiaea

Pi 0.244

MarkerSeek score 80.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 912
pi: 0.244
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCGTTGTGGTCGGACTCTAT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.2	58.0	2799-2832 bp	0.5	50.8
ndhF_p2	ATCAATTCGGTCGTTGTGGT	GAGGTAAGATCCGGTGAATCG	45.0	52.4	57.8	58.0	2809-2842 bp	0.5	50.7
ndhF_p3	TCTCTTCCTTCTCCGAGCTC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.2	58.0	2741-2774 bp	0.5	50.2
ndhF_p4	TCGTTGTGGTCGGACTCTAT	ACGATTTTGAAAGGCCTCAGA	50.0	42.9	58.2	57.9	2889-2922 bp	0.5	49.8
ndhF_p5	ATCAATTCGGTCGTTGTGGT	ACGATTTTGAAAGGCCTCAGA	45.0	42.9	57.8	57.9	2899-2932 bp	0.5	49.7