Hotspot

ndhE

SSC · 116709-117011 · 303 bp

Back to Dolomiaea

Pi 0.219

MarkerSeek score 66.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 110
pi: 0.219
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TCCTCTGTTCTTGGAGCAGA	ATTCTGCCTTTTCGTTGGGA	50.0	45.0	58.0	57.7	1328-1330 bp	0.5	57.8
ndhE_p2	TCCTCTGTTCTTGGAGCAGA	TCTGCCTTTTCGTTGGGATT	50.0	45.0	58.0	57.7	1326-1328 bp	0.5	57.8
ndhE_p3	TCCTCTGTTCTTGGAGCAGA	TTCTGCCTTTTCGTTGGGAT	50.0	45.0	58.0	57.7	1327-1329 bp	0.5	57.8
ndhE_p4	TCCTCTGTTCTTGGAGCAGA	TAGGGGGTCTGGGAGTAGTA	50.0	55.0	58.0	57.7	1367-1369 bp	0.5	57.7
ndhE_p5	TCCTCTGTTCTTGGAGCAGA	TAATCGGCGCAATTGCTGTA	50.0	45.0	58.0	58.3	929-931 bp	0.5	57.5