Hotspot

ndhD

SSC · 114605-116134 · 1530 bp

Back to Dolomiaea

Pi 0.255

MarkerSeek score 81.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 652
pi: 0.255
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAGTGATTCACGCGGTTCT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	1899-1900 bp	0.5	60.4
ndhD_p2	GTAGTGATTCACGCGGTTCT	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	2121-2122 bp	0.5	59.7
ndhD_p3	GTAGTGATTCACGCGGTTCT	TGATACCTTGGGACGGATGT	50.0	50.0	58.0	58.1	1937-1938 bp	0.5	59.5
ndhD_p4	GTAGTGATTCACGCGGTTCT	CTCGGGGAACTCATCGATTC	50.0	55.0	58.0	57.9	2117-2118 bp	0.5	59.3
ndhD_p5	GTAGTGATTCACGCGGTTCT	CAGAGGATTGTGTCGGTTGT	50.0	50.0	58.0	57.8	1886-1887 bp	0.5	59.2