Hotspot

ndhA

SSC · 118682-120834 · 2153 bp

Back to Dolomiaea

Pi 0.245

MarkerSeek score 80.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2153
variable_sites: 880
pi: 0.245
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	50.0	50.0	58.0	58.1	2734-2739 bp	0.5	59.4
ndhA_p2	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	50.0	50.0	58.0	58.1	2735-2740 bp	0.5	59.4
ndhA_p3	TGACCAATATACCTTGCGGC	TTATGCGAGAGTCGAAGCAC	50.0	50.0	58.0	58.1	2508-2513 bp	0.5	59.2
ndhA_p4	TGACCAATATACCTTGCGGC	TGGGAAGTCCAATGGCAAAA	50.0	45.0	58.0	57.9	2747-2752 bp	0.5	59.1
ndhA_p5	AAACGCTCGGATGTGATCAA	GGCAAAAAGAGGGGGATTCA	45.0	50.0	57.9	58.1	2821-2826 bp	0.5	58.9