Hotspot

ccsA

SSC · 113390-114358 · 969 bp

Back to Dolomiaea

Pi 0.197

MarkerSeek score 76.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 319
pi: 0.197
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	AGAACCGCGTGAATCACTAC	50.0	50.0	58.0	58.0	1296-1302 bp	0.5	60.2
ccsA_p2	TGAAATCGGTAGACACGCTG	AGAACCGCGTGAATCACTAC	50.0	50.0	58.0	58.0	1295-1301 bp	0.5	60.2
ccsA_p3	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1571-1578 bp	0.5	60.1
ccsA_p4	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1570-1577 bp	0.5	60.0
ccsA_p5	GTGAAATCGGTAGACACGCT	TAGCTTTTCGATGGCTTCCC	50.0	50.0	58.0	58.0	1794-1801 bp	0.5	60.0