Hotspot

ndhE-ndhG

SSC · 117012-117231 · 220 bp

Back to Dolomiaea

Pi 0.205

MarkerSeek score 72.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 220
variable_sites: 75
pi: 0.205
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCTCTGTTCTTGGAGCAGA	ATTCTGCCTTTTCGTTGGGA	50.0	45.0	58.0	57.7	1328-1330 bp	0.5	57.8
ndhE-ndhG_p2	TCCTCTGTTCTTGGAGCAGA	TCTGCCTTTTCGTTGGGATT	50.0	45.0	58.0	57.7	1326-1328 bp	0.5	57.8
ndhE-ndhG_p3	TCCTCTGTTCTTGGAGCAGA	TTCTGCCTTTTCGTTGGGAT	50.0	45.0	58.0	57.7	1327-1329 bp	0.5	57.8
ndhE-ndhG_p4	TCCTCTGTTCTTGGAGCAGA	TAGGGGGTCTGGGAGTAGTA	50.0	55.0	58.0	57.7	1367-1369 bp	0.5	57.7
ndhE-ndhG_p5	TCCTCTGTTCTTGGAGCAGA	TAATCGGCGCAATTGCTGTA	50.0	45.0	58.0	58.3	929-931 bp	0.5	57.5