Hotspot

rpl32-trnL-UAG

SSC · 112317-113168 · 852 bp

Back to Dolomiaea

Pi 0.196
MarkerSeek score 77.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
852
variable_sites
279
pi
0.196
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.73
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 984-1016 bp 0.5 59.7
rpl32-trnL-UAG_p2 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 985-1017 bp 0.5 59.7
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 967-999 bp 0.5 59.5
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 968-1000 bp 0.5 59.5
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG TGATCCACAAAGAAGAGCCG 50.0 50.0 57.6 57.9 1638-1671 bp 0.5 58.1