Hotspot

ycf1

SSC · 107462-112528 · 5067 bp

Back to Dolichoglottis

Pi 0.00178

MarkerSeek score 40.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5067
variable_sites: 9
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCGTCACCTATTTTTAGTAAGA	GGTCAGAAGAGGTTTTTCAAACA	34.8	39.1	55.1	57.4	196 bp	1.0	40.8
ycf1_p2	ACCGTCACCTATTTTTAGTAAGA	AGGTCAGAAGAGGTTTTTCAAAC	34.8	39.1	55.1	57.2	197 bp	1.0	40.8
ycf1_p3	ACCGTCACCTATTTTTAGTAAGAA	GGTCAGAAGAGGTTTTTCAAACA	33.3	39.1	55.8	57.4	196 bp	1.0	40.8
ycf1_p4	ACCGTCACCTATTTTTAGTAAGA	AGGTCAGAAGAGGTTTTTCA	34.8	40.0	55.1	54.1	197 bp	1.0	40.8
ycf1_p5	ACCGTCACCTATTTTTAGTAAGAAAC	GGTCAGAAGAGGTTTTTCAAACA	34.6	39.1	57.7	57.4	196 bp	1.0	40.8