Hotspot

rpl20-rps12

LSC · 68077-68823 · 747 bp

Back to Dolichoglottis

Pi 0.00269
MarkerSeek score 60.20
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
747
variable_sites
2
pi
0.00269
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCTCCGAGCGATATATCCCC CAGCGTCGAGGAACATGTAC 55.0 55.0 57.9 58.7 813-1369 bp 1.0 73.9
rpl20-rps12_p2 TCTCCGAGCGATATATCCCC AGCGTCGAGGAACATGTACT 55.0 50.0 57.9 58.8 812-1369 bp 1.0 73.1
rpl20-rps12_p3 TCTCCGAGCGATATATCCCC TCAGCGTCGAGGAACATGTA 55.0 50.0 57.9 58.8 814-1368 bp 1.0 73.1
rpl20-rps12_p4 TCTCCGAGCGATATATCCCC GCGTCGAGGAACATGTACTA 55.0 50.0 57.9 57.2 811-1368 bp 1.0 73.0
rpl20-rps12_p5 TTCTACGTCTCCGAGCGATA CAGCGTCGAGGAACATGTAC 50.0 55.0 57.8 58.7 820-1373 bp 1.0 72.5