Hotspot

psaI-pafII

LSC · 59058-59449 · 392 bp

Back to Dolichoglottis

Pi 0.00514

MarkerSeek score 57.20

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 2
pi: 0.00514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	GCAATCGCAATGGCTTCTTT	TACTTGTTTTTCGAGCCCCC	45.0	50.0	58.0	58.1	507-1371 bp	1.0	79.4
psaI-pafII_p2	GCAATCGCAATGGCTTCTTT	GGATAACAGCCCAGCAGAAA	45.0	50.0	58.0	57.9	528-1369 bp	1.0	79.1
psaI-pafII_p3	GCAATCGCAATGGCTTCTTT	AAAGGATAACAGCCCAGCAG	45.0	50.0	58.0	57.9	531-1371 bp	1.0	79.1
psaI-pafII_p4	TAGGCCTAGTATTTCCGGCA	TACTTGTTTTTCGAGCCCCC	50.0	50.0	57.9	58.1	524-1370 bp	1.0	78.6
psaI-pafII_p5	TAGGCCTAGTATTTCCGGCA	GGATAACAGCCCAGCAGAAA	50.0	50.0	57.9	57.9	545-1368 bp	1.0	78.4