Hotspot

trnE-UUC-rpoB

LSC · 12142-13098 · 957 bp

Back to Dolichoglottis

Pi 0.00314

MarkerSeek score 60.40

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 957
variable_sites: 3
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGAGTTGAACCCTCGTTTT	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.0	57.8	1098-1099 bp	1.0	78.0
trnE-UUC-rpoB_p2	GGGAGTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCCG	50.0	50.0	58.0	57.7	1099-1100 bp	1.0	78.0
trnE-UUC-rpoB_p3	TAACCACTGGACCATAGGGG	GTTCTTCTGTCAAACCCCGA	55.0	50.0	58.1	57.8	1057-1058 bp	1.0	77.3
trnE-UUC-rpoB_p4	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCCG	55.0	50.0	58.1	57.7	1058-1059 bp	1.0	77.3
trnE-UUC-rpoB_p5	CCCTCGTTTTCTCCGTGAAA	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.1	57.8	1088-1089 bp	1.0	77.2