Hotspot

trnC-GCA-petN

LSC · 9482-10302 · 821 bp

Back to Dolichoglottis

Pi 0.0049

MarkerSeek score 59.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 4
pi: 0.0049
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GTTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	944-953 bp	1.0	78.9
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	887-896 bp	1.0	78.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	905-914 bp	1.0	76.4
trnC-GCA-petN_p4	TGTTGTGTATCGTTTTGGCG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.3	58.2	945-954 bp	1.0	73.8
trnC-GCA-petN_p5	GTTGTGTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.0	57.1	943-952 bp	1.0	72.9