Hotspot

ndhF-rpl32

SSC · 116640-117663 · 1024 bp

Back to Dobinea

Pi 0.0128

MarkerSeek score 66.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1024
variable_sites: 13
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAAAAAGGA	AAAAAGCTTTCAACGCTGCC	45.0	45.0	58.0	58.4	1213-1220 bp	1.0	77.6
ndhF-rpl32_p2	TGTTGCTGTCGGAAAAAGGA	CTGCCCAATATCCCCCTTTT	45.0	50.0	58.0	57.5	1198-1205 bp	1.0	77.2
ndhF-rpl32_p3	TGTCGGAAAAAGGAGAAGCC	AAAAAGCTTTCAACGCTGCC	50.0	45.0	58.1	58.4	1207-1214 bp	1.0	77.1
ndhF-rpl32_p4	TGTCGGAAAAAGGAGAAGCC	CTGCCCAATATCCCCCTTTT	50.0	50.0	58.1	57.5	1192-1199 bp	1.0	76.7
ndhF-rpl32_p5	TGTTGCTGTCGGAAAAAGGA	TTCAACGCTGCCCAATATCC	45.0	50.0	58.0	58.6	1205-1212 bp	1.0	76.1