Hotspot

rps7-rps12

IRb · 101721-101773 · 53 bp

Back to Dobinea

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TGTAGTACCTCGACGTGACA	CTGTGGAAAGCCGTATTCGA	50.0	50.0	57.8	58.0	179 bp	1.0	79.1
rps7-rps12_p2	TGTAGTACCTCGACGTGACA	TCTGTGGAAAGCCGTATTCG	50.0	50.0	57.8	58.0	180 bp	1.0	79.1
rps7-rps12_p3	TGTAGTACCTCGACGTGACA	GGCTTGGAGGGAGATCTTTC	50.0	55.0	57.8	57.7	143 bp	1.0	76.5
rps7-rps12_p4	TGTAGTACCTCGACGTGACA	AGAATTCTGTGGAAAGCCGT	50.0	45.0	57.8	57.4	185 bp	1.0	74.5
rps7-rps12_p5	TCTGTAGTACCTCGACGTGA	CTGTGGAAAGCCGTATTCGA	50.0	50.0	57.3	58.0	181 bp	1.0	74.4