Hotspot

petA-psbJ

LSC · 66258-67358 · 1101 bp

Back to Dobinea

Pi 0.0109

MarkerSeek score 62.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 12
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.0	57.4	1243-1247 bp	1.0	75.9
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.2	57.4	1240-1244 bp	1.0	74.8
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCCTTGTA	50.0	50.0	58.0	57.2	1237-1241 bp	1.0	74.6
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.8	57.4	1208-1212 bp	1.0	74.5
petA-psbJ_p5	TTGGCATCTGTTGTTTTGGC	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.8	57.4	1211-1215 bp	1.0	74.4