Hotspot

rbcL-accD

LSC · 59685-60313 · 629 bp

Back to Dobinea

Pi 0.00637

MarkerSeek score 62.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 4
pi: 0.00637
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACCATCGAATTGAACCACCA	55.0	45.0	58.0	57.4	748-785 bp	1.0	75.6
rbcL-accD_p2	AAATGGAGTCCTGAACTGGC	ACCATCGAATTGAACCACCA	50.0	45.0	57.8	57.4	739-776 bp	1.0	74.3
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	GGTCCTCGCTTACACTGGTA	55.0	55.0	58.0	58.8	837-874 bp	1.0	73.6
rbcL-accD_p4	GGCTAGCAAATGGAGTCCTG	ACCATCGAATTGAACCACCA	55.0	45.0	58.3	57.4	746-783 bp	1.0	73.3
rbcL-accD_p5	GTGAGGCTAGCAAATGGAGT	ACCATCGAATTGAACCACCA	50.0	45.0	57.6	57.4	750-787 bp	1.0	72.6