Hotspot

trnM-CAU-psbD

LSC · 33949-35513 · 1565 bp

Back to Dobinea

Pi 0.00924

MarkerSeek score 58.90

Primer available yes

Label trnM-CAU-psbD

Feature Metadata

label_name: trnM-CAU-psbD
feature_type: igs
region: LSC
length_bp: 1565
variable_sites: 14
pi: 0.00924
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAAACGGTCCCTTCGTAACC	55.0	50.0	58.1	58.1	1663-1700 bp	1.0	81.6
trnM-CAU-psbD_p2	TCAGTGGTAGAGTAACGCCA	AAAACGGTCCCTTCGTAACC	50.0	50.0	58.1	58.1	1666-1703 bp	1.0	81.4
trnM-CAU-psbD_p3	GTGGTAGAGTAACGCCATGG	AAACAAAACGGTCCCTTCGT	55.0	45.0	58.1	58.2	1667-1704 bp	1.0	80.6
trnM-CAU-psbD_p4	TCAGTGGTAGAGTAACGCCA	AAACAAAACGGTCCCTTCGT	50.0	45.0	58.1	58.2	1670-1707 bp	1.0	80.4
trnM-CAU-psbD_p5	GTGGTAGAGTAACGCCATGG	CCCTTCGTAACCAGTCATCC	55.0	55.0	58.1	57.7	1655-1692 bp	1.0	80.1