Hotspot

trnE-UUC-trnM-CAU

LSC · 33211-33889 · 679 bp

Back to Dobinea

Pi 0.00737

MarkerSeek score 63.10

Primer available yes

Label trnE-UUC-trnM-CAU

Feature Metadata

label_name: trnE-UUC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 5
pi: 0.00737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnM-CAU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	813-814 bp	1.0	79.3
trnE-UUC-trnM-CAU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	776-777 bp	1.0	79.2
trnE-UUC-trnM-CAU_p3	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	792-793 bp	1.0	78.8
trnE-UUC-trnM-CAU_p4	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	755-756 bp	1.0	78.8
trnE-UUC-trnM-CAU_p5	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	789-790 bp	1.0	78.6