Hotspot

rps16-trnQ-UUG

LSC · 6628-7928 · 1301 bp

Back to Dobinea

Pi 0.0108

MarkerSeek score 64.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1301
variable_sites: 14
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1431-1513 bp	1.0	79.6
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1442-1524 bp	1.0	79.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1374-1455 bp	1.0	79.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GCTATCGGAGGTTCGAATCC	45.0	55.0	58.1	57.9	1381 bp	0.5	58.3
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GCTATCGGAGGTTCGAATCC	50.0	55.0	58.2	57.9	1392 bp	0.5	57.8