Hotspot

ycf1

SSC · 128292-133868 · 5577 bp

Back to Distylium

Pi 0.00125

MarkerSeek score 50.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5577
variable_sites: 39
pi: 0.00125
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATCGGTATGACCCATGGT	TGGATATGGCGAAAAGTCCG	50.0	50.0	58.2	58.1	351 bp	1.0	79.4
ycf1_p2	TGATCGGTATGACCCATGGT	TGAGGCATGGATCAATAGCG	50.0	50.0	58.2	57.8	272 bp	1.0	78.8
ycf1_p3	TGATCGGTATGACCCATGGT	GATATGGCGAAAAGTCCGGA	50.0	50.0	58.2	57.8	349 bp	1.0	77.9
ycf1_p4	TGATCGGTATGACCCATGGT	ATGGCGAAAAGTCCGGATAG	50.0	50.0	58.2	57.8	346 bp	1.0	77.9
ycf1_p5	TGATCGGTATGACCCATGGT	GGCGAAAAGTCCGGATAGAA	50.0	50.0	58.2	57.7	344 bp	1.0	77.4