Hotspot

rpoC2

LSC · 17836-21999 · 4164 bp

Back to Distylium

Pi 0.000495

MarkerSeek score 44.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4164
variable_sites: 8
pi: 0.000495
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AAAGCAGGATGTCCATGACG	CCTGGTCGAATTGGGAGAAG	50.0	55.0	58.3	58.0	207 bp	1.0	78.5
rpoC2_p2	AAAGCAGGATGTCCATGACG	GTCGAATTGGGAGAAGCTGT	50.0	50.0	58.3	57.9	203 bp	1.0	77.9
rpoC2_p3	GGATGTCCATGACGTGTACG	CCTGGTCGAATTGGGAGAAG	55.0	55.0	58.4	58.0	201 bp	1.0	77.0
rpoC2_p4	AAAGCAGGATGTCCATGACG	TGGTCGGAGTCCTACTCATG	50.0	55.0	58.3	58.2	231 bp	1.0	76.7
rpoC2_p5	GGATGTCCATGACGTGTACG	GTCGAATTGGGAGAAGCTGT	55.0	50.0	58.4	57.9	197 bp	1.0	76.5