Hotspot

atpF

LSC · 12826-14118 · 1293 bp

Back to Distylium

Pi 0.00082

MarkerSeek score 52.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1293
variable_sites: 3
pi: 0.00082
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	GAGGGGCGAAGTGATACAAA	50.0	50.0	57.7	57.6	1437-1443 bp	1.0	74.9
atpF_p2	GGCTCGAATGGTTACCATGA	AAAGGAGGGGCGAAGTGATA	50.0	50.0	57.7	58.4	1441-1447 bp	1.0	74.7
atpF_p3	GGCTCGAATGGTTACCATGA	TAAAACAAAGGAGGGGCGAA	50.0	45.0	57.7	57.3	1447-1453 bp	1.0	72.8
atpF_p4	GGCTCGAATGGTTACCATGA	AAAACAAAGGAGGGGCGAAG	50.0	50.0	57.7	58.7	1446-1452 bp	1.0	72.7
atpF_p5	ACTAATTTCGTCGGCTCGAA	GAGGGGCGAAGTGATACAAA	45.0	50.0	57.4	57.6	1449-1455 bp	1.0	72.4