Hotspot

rps15-ycf1

SSC · 127915-128291 · 377 bp

Back to Distylium

Pi 0.00338

MarkerSeek score 51.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 377
variable_sites: 6
pi: 0.00338
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCCCTGTTTTCTTCTTGCGA	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.4	57.9	477-485 bp	1.0	79.4
rps15-ycf1_p2	TCCCCTGTTTTCTTCTTGCG	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.4	57.9	478-486 bp	1.0	79.4
rps15-ycf1_p3	CCCCTGTTTTCTTCTTGCGA	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.4	57.9	476-484 bp	1.0	79.4
rps15-ycf1_p4	TCCCCTGTTTTCTTCTTGCG	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.4	57.9	477-485 bp	1.0	79.4
rps15-ycf1_p5	TCCAAATGTGAAGTCAGCCT	TGGCAGTCGTTTCAGTATGG	45.0	50.0	57.0	57.9	540-548 bp	0.916667	70.0