Hotspot

rpl32-trnL-UAG

SSC · 117537-118595 · 1059 bp

Back to Distylium

Pi 0.00167

MarkerSeek score 56.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1059
variable_sites: 9
pi: 0.00167
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCGTTAAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	1183-1193 bp	1.0	79.0
rpl32-trnL-UAG_p2	GGGCAGCGTTAAAAGCTTTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.9	57.8	1176-1186 bp	1.0	78.5
rpl32-trnL-UAG_p3	GGGCAGCGTTAAAAGCTTTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.9	57.8	1182-1192 bp	1.0	78.4
rpl32-trnL-UAG_p4	GGGCAGCGTTAAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.9	57.8	1181-1191 bp	1.0	78.4
rpl32-trnL-UAG_p5	AAAAGGAAGGGGTATTGGGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	1199-1209 bp	1.0	78.2