Pi
0.00184
MarkerSeek score
52.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1075-1085 bp | 1.0 | 80.5 |
| trnT-UGU-trnL-UAA_p2 | AAAGCCAGCTATCGGAATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1120-1130 bp | 1.0 | 80.4 |
| trnT-UGU-trnL-UAA_p3 | AAAGCCAGCTATCGGAATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1128-1138 bp | 1.0 | 79.9 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1083-1093 bp | 1.0 | 79.9 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1107-1117 bp | 1.0 | 79.5 |