Hotspot

petN-psbM

LSC · 30362-31542 · 1181 bp

Back to Distylium

Pi 0.000808

MarkerSeek score 52.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1181
variable_sites: 3
pi: 0.000808
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.1	57.6	1295-1306 bp	1.0	68.8
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1288-1299 bp	1.0	68.6
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1287-1298 bp	1.0	68.6
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1286-1297 bp	1.0	68.6
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1333-1344 bp	1.0	68.4