Hotspot

rps16-trnQ-UUG

LSC · 6251-7920 · 1670 bp

Back to Distylium

Pi 0.00129

MarkerSeek score 47.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1670
variable_sites: 10
pi: 0.00129
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1742-1752 bp	1.0	80.4
rps16-trnQ-UUG_p2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1744-1754 bp	1.0	79.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1735-1745 bp	1.0	79.3
rps16-trnQ-UUG_p4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1745-1755 bp	1.0	78.7
rps16-trnQ-UUG_p5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.4	58.1	1743-1753 bp	1.0	77.6