Hotspot

petB

LSC · 78490-79921 · 1432 bp

Back to Distyliopsis

Pi 0.00186

MarkerSeek score 60.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1432
variable_sites: 4
pi: 0.00186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGAACCACGAAGTCCTAGCT	ATTGTTCCTCCCCCAAATGA	50.0	45.0	58.1	56.7	1597 bp	1.0	69.5
petB_p2	AGAACCACGAAGTCCTAGCT	TTGTTCCTCCCCCAAATGAT	50.0	45.0	58.1	56.7	1596 bp	1.0	69.5
petB_p3	TCACAAGAACCACGAAGTCC	ATTGTTCCTCCCCCAAATGA	50.0	45.0	57.8	56.7	1602 bp	1.0	68.2
petB_p4	TCACAAGAACCACGAAGTCC	TTGTTCCTCCCCCAAATGAT	50.0	45.0	57.8	56.7	1601 bp	1.0	68.2
petB_p5	CACAAGAACCACGAAGTCCT	TTGTTCCTCCCCCAAATGAT	50.0	45.0	57.8	56.7	1600 bp	1.0	68.1