Hotspot

rps15-ycf1

SSC · 127917-128293 · 377 bp

Back to Distyliopsis

Pi 0.0106

MarkerSeek score 57.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 377
variable_sites: 6
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCCCTGTTTTCTTCTTGCGA	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.4	57.9	469-485 bp	1.0	79.8
rps15-ycf1_p2	TCCCCTGTTTTCTTCTTGCG	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.4	57.9	470-486 bp	1.0	79.8
rps15-ycf1_p3	CCCCTGTTTTCTTCTTGCGA	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.4	57.9	468-484 bp	1.0	79.8
rps15-ycf1_p4	TCCCCTGTTTTCTTCTTGCG	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.4	57.9	469-485 bp	1.0	79.7
rps15-ycf1_p5	CCCTGTTTTCTTCTTGCGAA	TGGCAGTCGTTTCAGTATGG	45.0	50.0	56.6	57.9	468-484 bp	1.0	71.4