Hotspot

petD-rpoA

LSC · 81288-81477 · 190 bp

Back to Distyliopsis

Pi 0.0108

MarkerSeek score 58.30

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 3
pi: 0.0108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CATTTCGTCGTCCAGTAGCA	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.0	55.2	353-360 bp	1.0	60.0
petD-rpoA_p2	GGTTGGGTATTGGAGCAACA	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	296-303 bp	1.0	59.8
petD-rpoA_p3	TGGTTGGGTATTGGAGCAAC	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	297-304 bp	1.0	59.8
petD-rpoA_p4	CCAGTAGCAACAACCGTCTT	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	342-349 bp	1.0	59.1
petD-rpoA_p5	TTTCGTCGTCCAGTAGCAAC	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.2	55.2	351-358 bp	1.0	58.2