Hotspot

psbZ-trnG-UCC

LSC · 39084-39481 · 398 bp

Back to Distyliopsis

Pi 0.0168

MarkerSeek score 54.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 10
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	555-763 bp	1.0	79.0
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	553-761 bp	1.0	78.6
psbZ-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	50.0	50.0	57.8	57.3	528-736 bp	1.0	74.6
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	TACCATTCGACCATATCCGC	55.0	50.0	58.3	57.3	526-734 bp	1.0	74.2
psbZ-trnG-UCC_p5	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	549-757 bp	1.0	73.8