Hotspot

trnT-GGU-psbD

LSC · 34452-35812 · 1361 bp

Back to Distyliopsis

Pi 0.00441

MarkerSeek score 60.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1361
variable_sites: 9
pi: 0.00441
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1524-1550 bp	1.0	77.7
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	50.0	50.0	57.9	57.7	1538-1564 bp	1.0	77.6
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	TAGACCGGACCAACCTACAA	50.0	50.0	57.9	57.7	1540-1566 bp	1.0	77.2
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCAAC	50.0	55.0	57.9	58.3	1546-1572 bp	1.0	76.9
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1523-1549 bp	1.0	76.5