Hotspot

rpl22

LSC · 82957-83406 · 450 bp

Back to Distichlis

Pi 0.0178

MarkerSeek score 65.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 8
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	ACCAAGTCTGAAACCGAGTG	AAGTAAAGTGTAGGCGGGGA	50.0	50.0	57.8	58.4	578-579 bp	1.0	76.5
rpl22_p2	ACCAAGTCTGAAACCGAGTG	AGTAAAGTGTAGGCGGGGAA	50.0	50.0	57.8	58.4	577-578 bp	1.0	76.5
rpl22_p3	ACCAAGTCTGAAACCGAGTG	TGTAGGCGGGGAATAACCTT	50.0	50.0	57.8	58.4	570-571 bp	1.0	76.0
rpl22_p4	ACCAAGTCTGAAACCGAGTG	AAAGTAAAGTGTAGGCGGGG	50.0	50.0	57.8	57.5	579-580 bp	1.0	75.4
rpl22_p5	CCAAGTCTGAAACCGAGTGG	AGTAAAGTGTAGGCGGGGAA	55.0	50.0	58.5	58.4	576-577 bp	1.0	74.5