Hotspot

rpl16

LSC · 78546-81917 · 3372 bp

Back to Distichlis

Pi 0.202

MarkerSeek score 77.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 3372
variable_sites: 585
pi: 0.202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCGAATGCAATGAGAGAGCT	CGAAATTAGGAGATATGCGCC	50.0	47.6	58.0	57.0	1527 bp	0.5	43.6
rpl16_p2	TGAAATGAGAGAGCTCCCGA	CGAAATTAGGAGATATGCGCC	50.0	47.6	58.1	57.0	1543 bp	0.5	42.8
rpl16_p3	CCGAATGCAATGAGAGAGCT	AGGATTGATTTGCAGCAAAACA	50.0	36.4	58.0	57.7	884 bp	0.5	40.9
rpl16_p4	AATACAATGAGAGACGCCGC	CGAAATTAGGAGATATGCGCC	50.0	47.6	58.4	57.0	1566 bp	0.5	40.6
rpl16_p5	TGAGAGAGCTCCCGAATACA	CGAAATTAGGAGATATGCGCC	50.0	47.6	57.6	57.0	1580 bp	0.5	40.5