Hotspot

psaC-ndhE

SSC · 110120-110610 · 491 bp

Back to Distichlis

Pi 0.0172

MarkerSeek score 71.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 491
variable_sites: 8
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	623-647 bp	1.0	80.9
psaC-ndhE_p2	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	624-648 bp	1.0	80.9
psaC-ndhE_p3	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	50.0	50.0	57.9	58.3	625-649 bp	1.0	80.8
psaC-ndhE_p4	ACATCTGTTGGACAAGCTCG	AGCCGCTATTGGACTATCCA	50.0	50.0	58.2	58.3	629-653 bp	1.0	80.4
psaC-ndhE_p5	CATCTGTTGGACAAGCTCGT	AGCCGCTATTGGACTATCCA	50.0	50.0	58.2	58.3	628-652 bp	1.0	80.4