Hotspot

rpl14-rpl16

LSC · 78437-78545 · 109 bp

Back to Distichlis

Pi 0.119

MarkerSeek score 78.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 13
pi: 0.119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GCTCCACTGTTATCCGCTAC	AATGCCCATACGAAGCCAAT	55.0	45.0	58.1	57.9	196-224 bp	1.0	83.2
rpl14-rpl16_p2	GCTCCACTGTTATCCGCTAC	ATGCCCATACGAAGCCAATT	55.0	45.0	58.1	57.9	195-223 bp	1.0	83.2
rpl14-rpl16_p3	AGCTCCACTGTTATCCGCTA	AATGCCCATACGAAGCCAAT	50.0	45.0	58.2	57.9	197-225 bp	1.0	82.6
rpl14-rpl16_p4	AGCTCCACTGTTATCCGCTA	ATGCCCATACGAAGCCAATT	50.0	45.0	58.2	57.9	196-224 bp	1.0	82.6
rpl14-rpl16_p5	GCTCCACTGTTATCCGCTAC	TGCCCATACGAAGCCAATTT	55.0	45.0	58.1	58.2	194-222 bp	1.0	82.6