Hotspot

petD-rps11

LSC · 74816-76319 · 1504 bp

Back to Distichlis

Pi 0.0621

MarkerSeek score 79.80

Primer available yes

Label petD-rps11

Feature Metadata

label_name: petD-rps11
feature_type: igs
region: LSC
length_bp: 1504
variable_sites: 87
pi: 0.0621
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rps11_p1	CGAGTGGTACTTCTTTCCCG	TTCGTACAGTGGGTTTGCAA	55.0	45.0	58.0	58.0	1922-1927 bp	1.0	87.3
petD-rps11_p2	TCCTTTGGGGGATGGATCTT	TTCGTACAGTGGGTTTGCAA	50.0	45.0	58.0	58.0	2185-2190 bp	1.0	86.7
petD-rps11_p3	TTCCTTTGGGGGATGGATCT	TTCGTACAGTGGGTTTGCAA	50.0	45.0	58.0	58.0	2186-2191 bp	1.0	86.7
petD-rps11_p4	TCCTTTGGGGGATGGATCTT	AAAGTGCGTATTGGTTTGCG	50.0	45.0	58.0	58.0	2411-2416 bp	1.0	86.0
petD-rps11_p5	TTCCTTTGGGGGATGGATCT	AAAGTGCGTATTGGTTTGCG	50.0	45.0	58.0	58.0	2412-2417 bp	1.0	86.0