Hotspot

rps18-rpl20

LSC · 66677-66908 · 232 bp

Back to Distichlis

Pi 0.0308

MarkerSeek score 67.20

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 7
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AAGAAACAACAATCGGAGCA	ATGCTTGCACAAGTAGCTGT	40.0	45.0	55.9	58.1	339-344 bp	1.0	65.0
rps18-rpl20_p2	GGAGCATAAGTTCCGATTGTTG	ATGCTTGCACAAGTAGCTGT	45.5	45.0	57.7	58.1	325-330 bp	1.0	63.9
rps18-rpl20_p3	CAACAATCGGAGCATAAGTTCC	ATGCTTGCACAAGTAGCTGT	45.5	45.0	57.7	58.1	333-338 bp	1.0	63.8
rps18-rpl20_p4	AGAAACAACAATCGGAGCAT	ATGCTTGCACAAGTAGCTGT	40.0	45.0	55.6	58.1	338-343 bp	1.0	63.2
rps18-rpl20_p5	GGAGCATAAGTTCCGATTGT	ATGCTTGCACAAGTAGCTGT	45.0	45.0	55.5	58.1	325-330 bp	1.0	62.4