Hotspot

petA-psbJ

LSC · 60961-61864 · 904 bp

Back to Distichlis

Pi 0.0156

MarkerSeek score 68.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 904
variable_sites: 14
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	1048-1076 bp	1.0	67.2
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	ACGGTTCGTATTCTGGATTGG	55.0	47.6	58.8	58.1	1059-1087 bp	1.0	66.4
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	50.0	47.6	58.7	57.7	1046-1074 bp	1.0	65.8
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	50.0	52.4	58.7	57.6	1104-1132 bp	1.0	65.0
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	50.0	52.4	58.7	57.6	1105-1133 bp	1.0	65.0