Hotspot

ndhF-rpl32

SSC · 114893-115743 · 851 bp

Back to Dissochaeta

Pi 0.0268
MarkerSeek score 72.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
851
variable_sites
33
pi
0.0268
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCATTGGAAAAACGA TGAAAAAGCCTTCAACGCTG 40.0 45.0 56.7 57.5 1049-1065 bp 1.0 70.7
ndhF-rpl32_p2 TGTTGCCATTGGAAAAACGA GAAAAAGCCTTCAACGCTGT 40.0 45.0 56.7 57.5 1048-1064 bp 1.0 70.7
ndhF-rpl32_p3 TTGTTGCCATTGGAAAAACGA TGAAAAAGCCTTCAACGCTG 38.1 45.0 57.5 57.5 1050-1066 bp 1.0 68.5
ndhF-rpl32_p4 TTGTTGCCATTGGAAAAACGA GAAAAAGCCTTCAACGCTGT 38.1 45.0 57.5 57.5 1049-1065 bp 1.0 68.5
ndhF-rpl32_p5 TGTTGCCATTGGAAAAACGAT TGAAAAAGCCTTCAACGCTG 38.1 45.0 57.3 57.5 1049-1065 bp 1.0 66.8